The present invention relate to a method to increase the production level
of paclitaxel by changing the temperature during the plant cell culture.
According to the present invention, the production of the paclitaxel
comprises the following procedure: (i) cultivating the Taxus genus plant
cells in a medium at ca. 20 to 25.degree. C.; and (ii) when growth of the
plant cells has progressed sufficiently, changing the cultivation
temperature to ca. 26 to 32.degree. C. to continue the culture. The
present invention comprises also the method of increasing the paclitaxel
production by inoculating the cells at a high initial concentration and by
increasing the saccharide concentration in the medium: According to the
present invention, paclitaxel can be mass-produced conveniently and
therefore has an industrial application.
