Transgenic plants with enhanced chlorophyll content and salt tolerance

   
   

The present invention relates to novel nucleic acid construct comprising (a) an anti-sense gene of a sense gene encoding E. histolytica calcium binding protein or a portion of said anti-sense gene, wherein said sense gene is at least 90% similar to the nucleic sequence of SEQ ID No: 1, and wherein said portion of the anti-sense gene is of a size capable of disrupting translation of said calcium binding protein; and (b) a constitutive promoter and a nopaline synthase (nos) polyadenylation signal sequence both operatively linked to said gene or portion thereof; wherein said construct is useful for increasing the level of chlorophyll in plants, a transgenic plant containing said construct and a novel nucleic acid construct useful for developing stress-tolerant plants, comprising (a) a sense gene encoding E. histolytica calcium binding or an altered sense gene wherein said gene encodes proteins of a sequence having biological properties identical to the said sense gene, and (b) the constitutive promoter and the nopaline synthase (nos) polyadenylation signal sequence both operatively linked to said gene or portion thereof.

A invenção atual relaciona-se à novela que a construção do ácido nucleic compreendendo (a) anti-deteta o gene de um gene do sentido que codifica proteína obrigatória do cálcio do histolytica do E. ou uma parcela de dito anti-deteta o gene, wherein o gene dito do sentido é ao menos 90% similar à seqüência nucleic do no. SEQ do ID: 1. e wherein a parcela dita do anti-deteta gene é de um tamanho capaz de disrupting a tradução de proteína obrigatória do cálcio dito; e (b) um promoter constitutive e uma seqüência ambos do sinal do polyadenylation do synthase do nopaline (no.) ligados operativa a gene ou a parcela dita disso; wherein a construção dita é útil para aumentar o nível do chlorophyll nas plantas, uma planta transgenic que contêm a construção dita e um ácido nucleic da novela constroem útil para desenvolver plantas forç-tolerantes, compreendendo (a) um gene do sentido que codificam o emperramento ou um gene alterado do sentido wherein o gene dito codifica proteínas de uma seqüência que tem as propriedades biológicas idênticas ao gene dito do sentido, e (b) o promoter constitutive e a seqüência ambos do cálcio do histolytica do E. do sinal do polyadenylation do synthase do nopaline (no.) ligados operativa a gene dito ou repartem disso.

 
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