Tryptase substrates and assay for tryptase activity using same

   
   

The invention is directed to synthetic polypeptide substrates for tryptase enzymes and assays for tryptase activity that utilize the synthetic polypeptide substrates. The preferred synthetic polypeptide substrates are tetramers of the formula P4-P3-P2-P1, wherein the substrate is selected from the group consisting of P-R-N-K (SEQ. ID. NO: 2), P-K-N-K (SEQ. ID. NO: 3), P-R-N-R (SEQ. ID. NO: 4), P-K-N-R (SEQ. ID. NO: 5), P-A-N-K (SEQ. ID. NO: 6), and P-R-T-K (SEQ. ID. NO: 7).

L'invention est dirigée vers les substrats synthétiques de polypeptide pour des enzymes de tryptase et les analyses pour l'activité de tryptase qui utilisent les substrats synthétiques de polypeptide. Les substrats synthétiques préférés de polypeptide sont des tétramères de la formule P4-P3-P2-P1, où le substrat est choisi dans le groupe de P-R-N-K (SEQ. ID. NON : 2), P-K-N-K (SEQ. ID. NON : 3), P-R-N-R (SEQ. ID. NON : 4), P-K-N-R (SEQ. ID. NON : 5), P-A-N-K (SEQ. ID. NON : 6), et P-R-T-K (SEQ. ID. NON : 7)

 
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