Polynucleotides encoding SREB3 receptor

   
   

This invention belongs to the genetic engineering field, and provides novel G protein-coupled receptor family proteins SREB1, SREB2 and SREB3 expressed in the central nervous system, genes coding for these proteins, screening methods using these proteins and so on. As one of the methods for obtaining the G protein-coupled receptor proteins of the present invention, RT-PCR is carried out using mRNA extracted from human or rat brain tissue or brain-derived cells as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, thereby obtaining cDNA corresponding to the G protein-coupled receptor protein or a part thereof, and the cDNA is integrated into an appropriate expression vector and expressed in a host cell.

_ este invenção pertenç genetic engenharia campo, e forneç novela g proteína-acopl receptor família proteína SREB1, SREB2 e SREB3 express central nervoso sistema, genes coding para este proteína, seleção método using este proteína e assim por diante so on. _ como um método para obtaining g proteína-acopl receptor proteína atual invenção, RT-PCR est realiz using mRNA extracted humano ou rato cérebro tecido ou cérebro-deriv pilha como molde e using dois primers interposing inteiro parcela ou um peça g proteína-acopl receptor proteína tradução região, desse modo obtaining dNA corresponding g proteína-acopl receptor proteína ou um peça disso, e dNA est integrated um apropriado expressão vetor e express um anfitrião pilha. _

 
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