A reconfigurable multi-mode post-column analysis system improves resolution
of liquid chromatography detection data by providing a parking or MS/MS
detection mode in which flow of column eluent is substantially reduced to
provide greater analysis time within a single eluting detection peak. A
micro switching valve unit is reconfigurable between normal (MS) mode that
passes column eluent directly to an MS unit, and a peak parking (or MS/MS)
mode, in which normal mode column flow is interrupted, and the gradient
halted, while more slowly flowing the detection peak of interest through
the MS unit. A micro syringe pump contributes to the substantially lower
MS/MS flow rate. A MS control unit controls micro switching valve valves
to reconfigure between MS and MS/MS modes. After a detection peak, MS mode
is reestablished, and the system primary pump returns to normal mode flow
rate and gradient conditions. Mode changes can be carried out for each
chromatogram detection peak.
