The invention discloses a method for producing a hybridization complex
whose stability is substantially independent of the base composition of
two hybridized nucleic acid molecules, consisting in contacting a first
nucleic acid molecule with a second nucleic acid molecule under conditions
enabling the formation of a specific hybridization complex. The invention
is characterized in that at least one of the four types of bases contained
in the sequence of the first and/or of the second nucleic acid molecule is
a modified base having pairing properties close to those of the natural
base it replaces, such that at least one of the two types of the base pair
contained in the composition of the complex comprises a modified base and
in that the totality of the base pairs contained in the composition of the
said complex has a like stability.
_ invenção divulg um método para producing um hybridization complexo cujo estabilidade est substancial independente base composição dois hybridized nucleic ácido molécula, consisting contacting um primeiro nucleic ácido molécula com um segundo nucleic ácido molécula sob condição enabling formação um específico hybridization complexo. _ invenção est caracteriz que ao menos um quatro tipo base contained seqüência primeiro e/ou segundo nucleic ácido molécula est um modific base t pairing propriedade perto aquele natural base substitu, tal que ao menos um dois tipo base par contained composição complexo compreend um modific base e que totality base par contained composição dito complexo t um como estabilidade. _
