Recombinant microbial strains are provided that express nitrilase enzyme
and are useful as biocatalysts for the hydrolysis of nitrile-containing
substrates. The recombinant cells are transformed with a foreign gene
isolated from Acidovorax facilis 72W encoding a thermostable nitrilase
enzyme that catalyzes the hydrolysis of nitrile-containing substrates to
carboxylic acids under mild reaction conditions. The nucleotide sequence
of the nitrilase gene and the deduced amino acid sequence encoded by the
nitrilase gene are provided.
As tensões microbial recombinant são contanto que enzyme expresso do nitrilase e são úteis como biocatalysts para o hydrolysis de carcaças nitrile-containing. As pilhas recombinant são transformadas com um gene extrangeiro isolado dos facilis 72W de Acidovorax que codificam um enzyme thermostable do nitrilase que catalyzes o hydrolysis de carcaças nitrile-containing aos ácidos carboxylic sob circunstâncias suaves da reação. A seqüência do nucleotide do gene do nitrilase e a seqüência deduzida do amino-ácido codificada pelo gene do nitrilase são fornecidas.
