This invention relates to the use of promoters for ribonucleic acid
amplification and other genetic manipulations. Processes are provided
wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a
ribonucleic acid (RNA) sequence using a complementary primer linked to an
RNA polymerase promoter region complement and then anti-sense RNA (aRNA)
is transcribed from the cDNA by introducing an RNA polymerase capable of
binding to the promoter region. Additional processes using the resulting
aRNA are also described.