This invention is directed to a process for tightly binding nucleic acid to
solid phase and corresponding processes for the utilization thereof.
Nucleic acid is bound to solid phase matrices exhibiting sufficient
hydrophilicity and electropositivity to tightly bind the nucleic acids
from a sample. These processes include nucleic acid (double or single
stranded DNA and RNA) capture from high volume and/or low concentration
specimens, buffer changes, washes, and volume reductions, and enable the
interface of solid phase bound nucleic acid with enzyme, hybridization or
amplification strategies. The tightly bound nucleic acid may be used, for
example, in repeated analyses to confirm results or test additional genes
in both research and commercial applications. Further, a method is
described for virus extraction, purification, and solid phase
amplification from large volume plasma specimens.
