Method for quickly detecting microbial dna/rna, kit therefor and the use of said method

   
   

A method for identifying microbial DNA/RNA comprising providing a sample containing microbial DNA/RNA; enriching DNA/RNA in the sample by a PCR method wherein the DNA/RNA that is identified comprise copies which are amplified by the PCR method; preliminarily classifying at least one microorganism by identifying the microbial DNA/RNA by a melting curve; adding at least one labeled oligonucleotide whereby temperature-dependent hybridization takes place and identifying the DNA/RNA on the basis of a physical property of the resultant DNA/RNA oligonucleotide complex, such as the temperature-dependence of the hybridization.

Un método para identificar DNA/RNA microbiano que abarca proporcionando una muestra que contiene DNA/RNA microbiano; enriquecer DNA/RNA en la muestra por un método de PCR en donde los DNA/RNA se identifican que abarcan las copias que son amplificadas por el método de PCR; preliminar clasificando por lo menos un microorganismo identificando el DNA/RNA microbiano por una curva que derrite; la adición por lo menos de uno etiquetó el oligonucleotide por el que ocurra el hibridación temperatura-dependiente e identificando el DNA/RNA en base de una característica física del complejo resultante del oligonucleotide de DNA/RNA, tal como la temperatura-dependencia del hibridación.

 
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