In general, the invention features methods and uses for
transposon-mediated gene targeting which greatly enhance the insertion
and detection of desired genes in genomic exons by homologous
recombination. The invention also features transgenic non-human mammals,
and eukaryotic cells, wherein a gene encoding 7B2 protein is modified, as
well as nucleic acid vectors capable of undergoing homologous
recombination with an endogenous 7B2 gene in a cell. The invention also
features transgenic non-human mammals as models of endocrine disorders,
as well as methods for diagnosing and treating patients with endocrine
disorders.
No general, a invenção caracteriza métodos e usos para a escolha de objectivos transposon-mediada do gene que realçam extremamente a inserção e a deteção de genes desejados em exons genomic pelo recombination homologous. A invenção caracteriza também mamíferos non-human transgenic, e pilhas eukaryotic, wherein um gene que codifica a proteína 7B2 é modificado, as.well.as os vetores do ácido nucleic capazes de submeter-se ao recombination homologous com um gene 7B2 endogenous em uma pilha. A invenção caracteriza também mamíferos non-human transgenic como modelos de disorders do endocrine, as.well.as métodos para diagnosticar e tratar pacientes com os disorders do endocrine.
