An object of the present invention is to provide a novel fluorescent
protein derived from organisms other than Aequorea Victoria. According to
the present invention, there is provided a fluorescent protein derived
from Fungia sp., which has the following properties:
(1) the excitation maximum wavelength is 455 nm, and the fluorescence
maximum wavelength is 488 nm;
(2) the molar absorption coefficient at 455 nm is 38700 or 27700;
(3) the quantum yield is 0.85 or 0.81; and
(4) the pH sensitivity of the fluorescent property is stable at pH 5 to 9;
and a fluorescent protein derived from Fungia sp., which has the
following properties:
(1) the excitation maximum wavelength is 548 nm, and the fluorescence
maximum wavelength is 561 nm;
(2) the molar absorption coefficient at 548 nm is 75900 or 51000;
(3) the quantum yield is 0.44 or 0.50; and
(4) the pH sensitivity of the fluorescent property is pKa<5.0.
