The present invention relates to a new plasmid originated from (Bifidobacterium)
a recombinant expression vector and transformation method using the same. More
particularly, the present invention relates to a plasmid pMG1 having nucleotide
sequence represented by SEQ.ID.NO.1; (Bifidobacterium longum) MG1 including
the plasmid pMG1; and a shuttle vector which can be replicated in both (Bifidobacterium)
and (E. coli), and comprises (Mob) gene having nucleotide sequence represented
by SEQ.ID.NO.2. (Rep) gene having nucleotide sequence represented by SEQ.ID.NO.3
and a selection marker. The shuttle vector and the promoter of the present invention
can be used for expressing target gene without additional purification process.
The protein expressed from the target gene in (Bifidobacterium) can be added
to food, therefore, the protein can be used for preparing food additives or oral
vaccine, Furthermore, the potential and the possibilty of probiotics using (Bifidobacterium)
can promoted through the development of the shuttle vector.
