Disclosed is a device and methods for the rapid chemiluminescence assay
of surfaces to detect the presence of microbial contamination. The device and methods
are suitable for use by untrained personnel under the relatively harsh and variable
conditions found in the field, for example in fast food restaurants and other food
preparation areas. The chemiluminescence reaction that is the source of the analytical
signal in the disclosed assay device and method is preferably based on a luciferase/luciferin system.