The present invention provides the D-aminoacylase-encoding gene derived from
Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the
gene, and the homologues thereof. The D-aminoacylase of the present invention is
capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful
as a medicinal raw material or the like.