The present invention relates to methods for producing a heterologous polypeptide,
comprising: (a) cultivating a mutant of a parent filamentous fungal cell under
conditions conducive for the production of the heterologous polypeptide, wherein
(i) the mutant cell comprises a nucleic acid sequence encoding the heterologous
polypeptide and (ii) the mutant produces less of the cyclohexadepsipeptide than
the parent filamentous fungal cell when cultured under the same conditions; and
(b) isolating the heterologous polypeptide from the cultivation medium. The present
invention also relates to mutants of filamentous fungal cells and methods for obtaining
the mutant cells. The present invention also relates to isolated cyclohexadepsipeptide
synthetases and isolated nucleic acid sequences encoding the cyclohexadepsipeptide
synthetases. The present invention also relates to nucleic acid constructs, vectors,
and host cells comprising the nucleic acid sequences as well as methods for producing
the cyclohexadepsipeptide synthetases. The present invention further relates to
cyclohexadepsipeptides produced by the cyclohexadepsipeptide synthetases.
