The present invention relates, in general, to a biotechnological method for production
of (3S,3S) astaxanthin. In particular, the present invention relates to
a peptide having a -C-4-oxygenase activity; a DNA segment coding for this
peptide; an RNA segments coding for this peptide; a recombinant DNA molecule comprising
a vector and the DNA segment; a host cell or organism containing the above described
recombinant DNA molecule or DNA segment; and to a method of biotechnologically
producing (3S,3S) astaxanthin or a food additive containing (3S,3S)
astaxanthin, using the host.