The present invention provides compositions for RNA interference and
methods of use thereof. The present invention is based on the development
of promoters that can be used to regulate shRNA expression spatially (in
specific cells) and temporally (at specific times) in cells or transgenic
animals that express a recombinase. The compositions and methods of the
present invention feature regulatable promoters that allow for inhibition
of the expression of target alleles in a spatially and temporally
regulatable manner. Thus, the compositions of the present invention are
useful for investigating gene functions, both physiologic and pathologic,
in specific cell groups and in specific ages, in normal and disease
pathways. Functional and genomic and proteomic methods are featured.
Therapeutic methods are also featured.
