The present invention relates to an improved method for the identification and
optionally the characterisation of interacting molecules designed to detect positive
clones from the rather large numbers of false positive clones isolated by conventional
two-hybrid systems. The method of the invention relies on a novel combination of
selection steps used to detect clones that express interacting molecules from false
positive clones. The present invention provides for high-throughput interaction
screens for the reliable identification of interacting molecules, which in turn
can lead to the identification of substances inhibiting said interactions. Such
inhibitors can find their use in the formulation of a pharmaceutical composition.
The present invention further relates to kits useful for carrying out the method
of the invention.
