Procedure for controlling the enumeration of the absolute count of cells
(or other particles) present in a sample. The procedure consists of the following:
to prepare, in known quantities and proportions, a mixture or stock solution of
two or more populations of reference particles of differing characteristics; to
add a known quantity of this mixture of reference particles to a known volume of
the sample which contains the cells (or other particles) to be counted; to measure,
in a flow cytometer, the sample containing both the cells (or other particles),
the events of which are to be counted, and the mixture of different populations
of reference particles; to calculate the absolute number of cells (or other particles)
present in the sample to be counted and; to check that the proportion between the
different reference particles present in the sample measured concurs with the proportion
that exists between them in the initial mixture or stock solution which contained
the different populations of reference particles prior to adding it to the sample.
