This invention provides solid phase specific binding lateral flow assay methods,
devices and kits for quantitating high and low molecular weight analytes. The methods
and devices of the invention employ labelled reagents which are either analyte
analogs or complementary specific binding pair members for the analyte and a novel
arrangement of capture zones comprising immobilized specific binding substances
for either the analyte or the labelled reagent to effect bound from unbound labelled
reagent as a function of analyte concentration. The capture zones are disposed
on a non-bibulous matrix defining a flow path from a sample receiving zone to the
capture zone. The devices of this invention also include multilane flow paths and
multiple capture zones to quantitate analyte.
