Novel protease variants derived from the DNA sequences of naturally-occurring
or recombinant non-human proteases are disclosed. The variant proteases, in general,
are obtained by in vitro modification of a precursor DNA sequence encoding the
naturally-occurring or recombinant protease to generate the substitution of a plurality
of amino acid residues in the amino acid sequence of a precursor protease. Such
variant proteases have properties which are different from those of the precursor
protease, such as altered wash performance. The substituted amino acid residue
correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
