An isolated polynucleotide which contains a polynucleotide sequence selected
from
the group comprising:
- (a) a polynucleotide which is at least 70% identical to a polynucleotide
coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2,
- (b) a polynucleotide coding for a polypeptide containing an amino acid
sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2,
- (c) a polynucleotide which is complementary to the polynucleotides of
(a) or (b), and
- (d) a polynucleotide containing at least 15 consecutive nucleotides
of the polynucleotide sequence of (a), (b), or (c),
- and a fermentation process for the preparation of L-amino acids using
corynebacteria in which at least the pknD gene is amplified, and to the use, as
hybridization probes, of polynucleotides containing the sequences according to
the invention.
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