This invention describes methods for enhancing carbon flow into a pathway of
a host cell to enhance the biosynthetic production of compounds therefrom, the
host cells being selected based on being phenotypically Pts-/glucose+.
Such host cells are capable of transporting glucose without consuming PEP, resulting
in conservation of PEP which can be re-directed into the pathway in order to enhance
the production of desired compounds along the pathway. Pts-/glucose+
mutants have been shown to be advantageous for the enhanced production of the aromatic
amino acids.