An invention comprising cellular transformation, directed evolution, and screening
methods for creating novel transgenic organisms having desirable properties. Thus
in one aspect, this invention relates to a method of generating a transgenic organism,
such as a microbe or a plant, having a plurality of traits that are differentially
activatable. Also, a method of retooling genes and gene pathways by the introduction
of regulatory sequences, such as promoters, that are operable in an intended host,
thus conferring operability to a novel gene pathway when it is introduced into
an intended host. For example a novel man-made gene pathway, generated based on
microbially-derived progenitor templates, that is operable in a plant cell. Furthermore,
a method of generating novel host organisms having increased expression of desirable
traits, recombinant genes, and gene products.