An objective of the present invention is to provide a transformant altered
so as to produce a secondary metabolite in which a benzene ring of the
secondary product is modified at the para-position with a functional
group containing a nitrogen atom. A transformant according to the present
invention is a transformant of an organism producing a secondary
metabolite having a benzene ring skeleton without substitution at the
para-position with a functional group containing a nitrogen atom, said
transformant being transformed by introducing genes involved in a
biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid,
including a gene encoding an amino acid sequence (SEQ ID NO: 2) having
4-amino-4-deoxychorismic acid synthase activity, a gene encoding an amino
acid sequence (SEQ ID NO: 4) having 4-amino-4-deoxychorismic acid mutase
activity and a gene encoding an amino acid sequence (SEQ ID NO: 6) having
4-amino-4-deoxyprephenic acid dehydrogenase activity, so as to produce a
secondary metabolite having a benzene ring skeleton substituted at the
para-position with a functional group containing a nitrogen atom. Another
objective of the present invention is to provide a novel gene involved in
the biosynthetic pathway from chorismic acid to p-aminophenylpyruvic
acid. A novel gene according to the present invention comprises genes
encoding the amino acid sequences of SEQ ID NOs: 2, 4 and 6 or modified
sequences thereof.