The present invention provides methods to engineer microorganisms for the
production of C.sub.30-aldehyde carotenoids. Specifically, various
combinations of crtM, sqs, crtN and crtN2 genes from Staphylococcus
aureus and Methylomonas sp. 16a can be co-expressed in transformant
hosts, leading to the production of diaponeurosporene monoaldehyde,
diapocarotene monoaldehyde, and/or diapocarotene dialdehyde. In a
preferred embodiment, the genetically engineered pathway is introduced
into a strain of Escherichia coli that has been engineered for the
expression of carotenoids, and the C.sub.30-carotenoid product is
diapocarotene dialdehyde.