A method and apparatus for automated cell analysis of biological specimens
automatically scans at a low magnification to acquire images which are
analyzed to determine candidate cell objects of interest. The low
magnification images are converted from a first color space to a second
color space. The color space converted image is then low pass filtered
and compared to a threshold to remove artifacts and background objects
from the candidate object of interest pixels of the color converted
image. The candidate object of interest pixels are morphologically
processed to group candidate object of interest pixels together into
groups which are compared to blob parameters to identify candidate
objects of interest which correspond to cells or other structures
relevant to medical diagnosis of the biological specimen. The location
coordinates of the objects of interest are stored and additional images
of the candidate cell objects are acquired at high magnification. The
high magnification images are analyzed in the same manner as the low
magnification images to confirm the candidate objects of interest which
are objects of interest. A high magnification image of each confirmed
object of interest is stored for later review and evaluation by a
pathologist.