Methods for producing modified polypeptides containing amino acid
analogues are disclosed, as well as compositions comprising purified
dihydrofolate reductase polypeptides, produced by the methods of the
invention. In certain aspects, methionine residues of the compositions
are replaced with homoallyglycine, homoproparglycine, norvaline,
norleucine, cis-crotyiglycine, trans-crotylglycine, 2-aminoheptanoic
acid, 2-butynyiglycine and allyglycine.