The invention relates to optical microscopy, and more particularly to the
methods for photothermal examination of absorbing microheterogeneities
using laser radiation. The invention can be widely used in laser
technique, industry, and biomedicine to examine transparent objects with
absorbing submicron fragments, including detection of local impurities
and defects in super-pure optical and semiconducting materials and
non-destructive diagnostics of biological samples on cellular and
subcellular levels.The object of the present invention is to increase
sensitivity, spatial resolution and informative worth when examining
local absorbing heterogeneities in transparent objects, as well as to
detect the size of said heterogeneities even if said size is smaller than
the radiation wavelength used. Said object is achieved by the pump beam
irradiation of a sample, the duration of said irradiation not being
longer than the characteristic time of cooling of the microheterogeneity
observed. A relatively vast surface of the sample is irradiated at once,
the size of said surface not being larger than the wavelength of the pump
laser used. The refraction index thermal variations, induced by the pump
beam in the sample and being the result of absorption, are registered by
the parameter change of the probe laser beam. A chosen probe beam
diameter should not be smaller than the pump beam diameter. The
diffraction-limited phase distribution over the probe laser beam
cross-section is transformed to an amplitude image using a phase contrast
method. The properties of microheterogeneities are estimated by measuring
said amplitude image.