Aspects of the present invention provide systems and methods relating to online gaming utilizing virtual playing instruments generated from physical playing instruments. According to one aspect of the invention, physical playing instruments are utilized in a gaming environment that may be scrambled, shuffled, and/or played remotely over a network. In one embodiment, the physical playing instruments are traditional poker-style gaming playing cards. The playing instruments include at least one identifier that may be read upon being dealt, such as identified and stored on a computer-readable medium before a game. In one such embodiment, computer-executable instructions may utilize the information on the computer-readable medium in conjunction with one or more games. Further aspects relate to validating the playing instruments and/or systems before, during, and/or after conducting one or more games with the playing instruments.