The present invention relates to a herbal mixture extract of Notoginseng radix, Rehmanniae radix preparata and Acanthopanacis cortex, and a composition for the prevention and the treatment of arthritis comprising the same as an effective ingredient. The herbal mixture extract of the present invention can be effectively used for the prevention and the treatment of arthritis because it inhibits the release of an inflammatory substance and the synthesis of cartilage destroying substance as well as the progress of arthritis.