The present invention provides a method of standardization of chemical and therapeutic properties and quality of foods and medicines. The present invention provides a method of chromatographic finger printing facilitating correlation of traditional methods used for chemical and therapeutic standardization of medicines and humors in the living things with physico chemical properties of the medicines and their constituents. The method is used for the qualitative and quantitative analysis of the energy involved in the medicines and living things and to understand various bio chemical reactions in living things using an energy system. It provides a rational basis to understand the traditional methods of assessment of chemical and therapeutic qualities of materials used for the said purpose. The present invention also provides the influence, of factors like pH, temperature, viscosity and ionic nature of the media along with atomic and molecular properties indicating the chemical and therapeutic values of the foods and medicines of natural and synthetic nature. The analysis of biological samples like blood indicated the utility of the method for the assessment of clinical pathological conditions of healthy and diseased. This facilitates for a better drug discovery, drug monitoring, drug targeting and drug profiling using different features of 3-D energy box created after analyzing the sample by different analysis, separation and detection methods.