The present specification relates to methods for assessing the physiological activity of a target site being evaluated for potential administration of a Clostridial toxin, methods for administering a Clostridial toxin to a particular target site, methods for assessing the effect of an administration of a Clostridial toxin in a mammal and methods assessing the extent of dispersal of a Clostridial toxin from a target area to a non-target area in a mammal.