A power supply circuit for a first and a second lamps and the transformer thereof is provided. The power supply circuit comprises a driving circuit and a transformer. The transformer includes a magnetic core, a primary coil set, a secondary coil, a first coil and a second coil. The driving circuit provides an alternative voltage. The primary coil set is wound on the magnetic core, along with the secondary coil, the first and the second coils, and receives the alternative voltage. The turns of the first coil and the second coil are substantially the same. The first lamp is coupled between the first coil and the secondary coil, and the second lamp is coupled between the second coil and the secondary coil.