The present invention relates to the enzymatic synthesis of
oligosaccharides, including sialylated product saccharides. In
particular, it relates to the use of recombinant cells to take up low
cost precursors such as glucose, pyruvate and N-actyl-glucosamine, and to
synthesize activated sugar moieties that are used in oligosaccharide
synthesis. The methods make possible the synthesis of many
oligosaccharides using microorganisms and readily available, relatively
inexpensive starting materials.