The present invention relates to the use of Stefin A as a scaffold protein
for the display of inserted peptides, particularly wherein the Stefin A
is a human Stefin A. Several mutations are advantageously made in the
wild type stefin A sequence to improve it as a scaffold; preferably the
Stefin A comprises a heterologous peptide insertion at the Leu 73 site.
Furthermore, preferably the scaffold protein comprises a V48D mutation;
preferably the scaffold protein comprises a G4W mutation. Preferably the
scaffold comprises Leu73, V48D and G4W mutations. The invention also
relates to the scaffold proteins themselves, in particular a stefin A
polypeptide having the Leu73, V48D and G4W mutations, such as shown as
SEQ ID NO: 1. The invention also relates to a method for identifying
binding proteins and to peptide A (RLNKPLPSLPV) and its use in treating
yeast infections.