A system and method for capturing, measuring and analyzing motion in real-time is provided which operates with or without markers. A threshold image is based on a background image and is created to minimize noise during the motion capture. A captured image is compared to the threshold image to identify collections of hot pixels or globs. The globs are compared to expected characteristics of the markers or the subject and are tracked between frames. Glob information is used to determine the location of the markers or subject in each frame.