An isolated mutant of coryneform bacteria comprising a gene encodes a
polypeptide having 2-methylcitrate dehydratase activity, where the
polypeptide comprises an amino acid sequence in which one of the
proteinogenic amino acids except L-proline is present at position 272 or
a corresponding or comparable position. In addition, an isolated
polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase
enzymic activity, which comprises at position 272 of the amino acid
sequence or a corresponding or comparable position a proteinogenic amino
acid except L-proline is described. A method for producing a recombinant
coryneform bacterium and L-amino acids. A recombinant microorganism,
L-Lysine-containing feed additive, and L-Tryptophan-containing feed
additive is also described.