process for the preparation of a material comprising conjugated isomers of
a polyunsaturated fatty acid comprises: treating a first fatty acid
mixture comprising saturated fatty acids, monounsaturated fatty acids and
polyunsaturated fatty acids in the presence of ethanol to form (i) a
solid fraction and (ii) a liquid fraction comprising a second fatty acid
mixture, wherein the second fatty acid mixture has a higher molar ratio
of total polyunsaturated fatty acids to total saturated and
monounsaturated fatty acids than the first fatty acid mixture; separating
the solid fraction and the liquid fraction; and subjecting the second
fatty acid mixture or a derivative or reaction product thereof to
treatment with a base in the presence of a solvent, to form conjugated
isomers of at least some of the polyunsaturated fatty acids.