An anisotropic conductive sheet interposed between a circuit board such as a substrate and various circuit parts to render them conductive and its manufacturing method. The anisotropic conductive sheet has a fine pitch required by the recent highly integrated circuit boards and electronic parts. In the anisotropic conductive sheet in which conductive members are scattered in a nonconductive matrix, the conductive members (e.g., 24) penetrate through the sheet (10) in the direction of thickness and conductive auxiliary layers (e.g., 25) are in contact with the conductive members (e.g., 24).