The present invention is directed to a cultured, stratified cartilage
tissue that comprises a tissue-engineered, cohesive cartilage construct
comprised of two or more cartilage layers, wherein each cartilage layer
comprises chondrogenic cells having a chondrocytic phenotype
corresponding to chondrocytes present in: a superficial-tangential zone
of natural cartilage; a middle-transitional zone of natural cartilage; a
deep-radial zone of natural cartilage; or a calcified cartilage zone of
natural cartilage.