The invention is directed to producing a shaped cartilage matrix isolated
from a human or animal where the cartilage has been crafted to facilitate
disinfection, cleaning, devitalization, recellularization, and/or
integration after implantation. The invention relates to a process for
repairing a cartilage defect and implantation of a cartilage graft into a
human or animal by crafting the cartilage matrix into individual grafts,
disinfecting and cleaning the cartilage graft, applying a pretreatment
solution to the cartilage graft, removing cellular debris using an
extracting solution to produce a devitalized cartilage graft, implanting
the cartilage graft into the cartilage defect with or without an
insertion device, and sealing the implanted cartilage graft with
recipient tissue. The devitalized cartilage graft is optionally
recellularized in vitro, in vivo, or in situ with viable cells to render
the tissue vital before or after the implantation. The devitalized
cartilage graft is also optionally stored between the removing cellular
debris and the recellularizing steps.