The present invention relates to a method for obtaining a transgenic
monocotyledon plant containing a gene of interest free of foreign
ancillary sequences comprising: a) transforming at least one cell of a
plant having no active transposase with a vector comprising two
expression cassettes, one comprising a nucleotide sequence of interest
(i), the other comprising a nucleotide sequence encoding a selection
marker (ii) bordered by the mobilizable sequences of a transposon, said
first expression cassette (comprising the nucleotide sequence of interest
(i)) being outside the transposon element; b) selecting the transformed
plants with the selection marker (ii); c) crossing a transformed plant
with another plant belonging to a line containing in its genome a gene
encoding an endogenous active transposase, and which is in the middle of
a phenotypic marker for excision (iii), to obtain an F1 or any other
individual of a subsequent generation; d) selecting the cells or the
individuals carrying the gene of interest free of foreign ancillary
sequence, from the F1 generation; e) regenerating plants from the cells
or the individuals selected in (d).