This invention is directed to a monopartite RNA viral vector comprising
modified tobravirus RNA-1 comprising an inserted foreign RNA sequence.
This invention is also directed to a bipartite RNA viral vector derived
from a tobravirus, wherein the vector comprises one or more foreign RNA
sequences. The invention is directed to a method of silencing one or more
endogenous plant host genes and a method of simultaneously silencing a
plant host gene and expressing a foreign gene in a plant host. Such
methods comprise infecting a plant host with a bipartite vector
comprising modified tobravirus RNA-1 and RNA-2. The invention is further
directed to a method of compiling a plant functional gene profile, a
method of changing the phenotype or biochemistry of a plant host, and a
method of determining the presence of a trait in a plant host, using a
monopartite or bipartite viral vector derived from a tobravirus.