A method of preparing a purified, virus inactivated and virus safe
antibody preparation from a starting solution comprising antibodies and
contaminants, the method comprising the steps of: (a) adjusting the pH of
the starting solution to about 4.6 to about 4.95 in particular to about
4.8 to about 4.95 to produce an intermediate solution; (b) adding
caprylate and/or heptanoate ions to the intermediate solution and
maintaining the pH at about 4.6 to about 4.95 in particular pH at about
4.8 to about 4.95, whereby a precipitate is formed and the antibodies are
essentially present in the supernatant; (c) incubating the supernatant
solution under conditions of caprylate and/or heptanoate ion
concentration, time, pH and temperature optionally concentrating and
diafiltrating the filtrated solution before pH adjustment; (d) applying
the filtered solution with a least one anion exchange resin and
optionally with two different anion exchange resins under conditions that
allow binding of contaminants to the resin while not allowing significant
binding' of the antibodies to the resin, wherein a purified, virus
inactivated and virus safe antibody preparation is produced.