One automated imaging process, as described herein, includes: a) obtaining
digital images of objects in a biological sample; b) selecting a
plurality of objects of interest from the digital images; c) obtaining
multiple images of the selected objects of interest at a plurality of
different wavelengths; d) combining one of said multiple images with a
corresponding digital image to produce a combined image; and e) analyzing
the combined image in order to characterize the biological sample.