A method for synchronizing at least one multimedia peripheral of a portable communication device with a synthetic audio file (1), which method comprises a step of generating at least one synchronization signal (S.sup.i.sub.SYNCH) from said synthetic audio file (1). According to the invention, said method comprises an initialization step (A.sub.0) consisting in memorizing, at said portable communication device, parameters (2) including at least a list of preferred instruments; and a generating step (A.sub.1) comprising a step of analyzing (A.sub.10) information read from said synthetic audio file (1) relating to each instrument for which a track can be extracted in said synthetic audio file (1), and a step of deciding (A.sub.11) which extracted track is to be used for generating said synchronization signal (S.sup.i.sub.SYNCH) as a function of said parameters. Said parameters (2) also include a predetermined frequency threshold, and said means for reading and analyzing are adapted to determine the frequency apparition of notes in said synthetic audio file for each extracted track.