Method of production of recombinant sucrose synthase, use thereof in the
manufacture of kits for determination of sucrose, production of
ADPglucose and production of transgenic plants whose leaves and storage
organs accumulate high contents of ADPglucose and starch
A method is described for efficient production of large quantities of
soluble recombinant SS in its active form, by expression of the gene that
encodes SS in a strain of Escherichia coli. The expression vector used
means that the recombinant SS produced possesses a histidine tail which
facilitates its quick purification. In addition it describes sequences of
mutated versions of the gene of SS that encode isoforms of SS suitable
for the production of ADPG. Making use of the "wild-type" and "mutated"
versions of recombinant SS, an efficient method is described for
production of ADPG and UDPG. It also describes the use of SS for the
production of assay kits for the determination of sucrose. Finally, it
describes the production of transgenic plants which overexpress the gene
of SS, either constitutively, or in leaves or storage organs, and which
have a high content (both in leaves and in storage tissues) of sucrose,
ADPG, G6P and starch as a result of the high ADPG-synthesizing activity
of SS.