Provided is a high-repetition-rate femtosecond regenerative amplification system. The regenerative amplification system includes: a laser oscillator emitting pulses; a stretcher stretching the pulses with negative dispersion; a regenerative amplifier amplifying the pulses, the regenerative amplifier comprising an acousto-optic modulator for pulse switching, a pulsed pump laser for pumping a gain medium, a resonator for reciprocating the pulses between a plurality of mirrors, and at least one chirped mirror for providing negative dispersion; and a glass compressor compressing the pulses. Accordingly, the 100 kHz-class high-repetition-rate femtosecond regenerative amplification system can produce an output energy of tens of .mu.J, higher than a few .mu.J provided by a conventional system.